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primary antibodies against ap4m1  (Proteintech)


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    Structured Review

    Proteintech primary antibodies against ap4m1
    Fig. 1 AP4M1is highly expressed in liver cancer. (A) <t>AP4M1</t> expression in various tumors in TCGA dataset. (B) The GEO dataset and TCGA-LIHC dataset download from the BEST database showed that AP4M1 was highly expressed in HCC. (C) AP4M1 protein expression levels obtained from CPTAC database. (D) AP4M1 protein levels from the Pan-cancer proteomics study
    Primary Antibodies Against Ap4m1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+antibodies+against+ap4m1/AP4M1+Antibody/pm37821948-85-0-10
    Average 93 stars, based on 2 article reviews
    primary antibodies against ap4m1 - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "AP4M1 as a prognostic biomarker associated with cell proliferation, migration and immune regulation in hepatocellular carcinoma."

    Article Title: AP4M1 as a prognostic biomarker associated with cell proliferation, migration and immune regulation in hepatocellular carcinoma.

    Journal: Cancer cell international

    doi: 10.1186/s12935-023-03089-0

    Fig. 1 AP4M1is highly expressed in liver cancer. (A) AP4M1 expression in various tumors in TCGA dataset. (B) The GEO dataset and TCGA-LIHC dataset download from the BEST database showed that AP4M1 was highly expressed in HCC. (C) AP4M1 protein expression levels obtained from CPTAC database. (D) AP4M1 protein levels from the Pan-cancer proteomics study
    Figure Legend Snippet: Fig. 1 AP4M1is highly expressed in liver cancer. (A) AP4M1 expression in various tumors in TCGA dataset. (B) The GEO dataset and TCGA-LIHC dataset download from the BEST database showed that AP4M1 was highly expressed in HCC. (C) AP4M1 protein expression levels obtained from CPTAC database. (D) AP4M1 protein levels from the Pan-cancer proteomics study

    Techniques Used: Expressing

    Fig. 3 Prognostic value ofAP4M1in HCC. (A) Overall survival analysis of AP4M1 mRNA high and low expression in HCC. (B) DSS analysis of AP4M1 mRNA high and low expression in HCC (C)PFS analysis of AP4M1 mRNA high and low expression in HCC (D) RFS analysis of AP4M1 mRNA high and low expres sion in HCC. (E) Survival curves of high and low AP4M1 expression in the GSE54236. (F) Time-dependent ROC curve. (G) Univariate and (H) multivariate COX regression analysis of OS correlation in HCC. OS: overall survival; PFS: Progression Free Survival; RFS: Recurrence Free Survival; DSS: Disease Specific Survival; HCC: hepatocellular carcinoma
    Figure Legend Snippet: Fig. 3 Prognostic value ofAP4M1in HCC. (A) Overall survival analysis of AP4M1 mRNA high and low expression in HCC. (B) DSS analysis of AP4M1 mRNA high and low expression in HCC (C)PFS analysis of AP4M1 mRNA high and low expression in HCC (D) RFS analysis of AP4M1 mRNA high and low expres sion in HCC. (E) Survival curves of high and low AP4M1 expression in the GSE54236. (F) Time-dependent ROC curve. (G) Univariate and (H) multivariate COX regression analysis of OS correlation in HCC. OS: overall survival; PFS: Progression Free Survival; RFS: Recurrence Free Survival; DSS: Disease Specific Survival; HCC: hepatocellular carcinoma

    Techniques Used: Expressing

    Fig. 4 AP4M1 genetic alterations in HCC. (A) Types of AP4M1 mutation in HCC. (B) AP4M1 alteration was associated with overall survival in HCC. (C) AP4M1 alteration was associated with disease-free survival in HCC. (D) Somatic landscape of HCC in AP4M1-high and AP4M1-low subgroup
    Figure Legend Snippet: Fig. 4 AP4M1 genetic alterations in HCC. (A) Types of AP4M1 mutation in HCC. (B) AP4M1 alteration was associated with overall survival in HCC. (C) AP4M1 alteration was associated with disease-free survival in HCC. (D) Somatic landscape of HCC in AP4M1-high and AP4M1-low subgroup

    Techniques Used: Mutagenesis

    Fig. 5 Association betweenAP4M1and immune cell infiltration in HCC. (A) AP4M1 is correlated with immune infiltration in HCC. (B-M) According to different expression levels of AP4M1, the infiltration levels of immune cells were analyzed in groups. (N) The relationship between the altered somatic copy number of AP4M1 gene and infiltrating immune cells in HCC. (O) The expression of AP4M1 in immune subtypes of liver cancer. (* p < 0.05; ** p < 0.01; *** p < 0.001, ns, no significance)
    Figure Legend Snippet: Fig. 5 Association betweenAP4M1and immune cell infiltration in HCC. (A) AP4M1 is correlated with immune infiltration in HCC. (B-M) According to different expression levels of AP4M1, the infiltration levels of immune cells were analyzed in groups. (N) The relationship between the altered somatic copy number of AP4M1 gene and infiltrating immune cells in HCC. (O) The expression of AP4M1 in immune subtypes of liver cancer. (* p < 0.05; ** p < 0.01; *** p < 0.001, ns, no significance)

    Techniques Used: Expressing

    Fig. 6 The correlation analysis ofAP4M1expression and immune checkpoint genes. (A) Immunostimulatory factors (B)Immunosuppressive factors. (C-G) Diagnostic value of AP4M1 for differentiating immunotherapy responses in an immunotherapy cohort. (* p < 0.05; ** p < 0.01; *** p < 0.001, ns, no significance)
    Figure Legend Snippet: Fig. 6 The correlation analysis ofAP4M1expression and immune checkpoint genes. (A) Immunostimulatory factors (B)Immunosuppressive factors. (C-G) Diagnostic value of AP4M1 for differentiating immunotherapy responses in an immunotherapy cohort. (* p < 0.05; ** p < 0.01; *** p < 0.001, ns, no significance)

    Techniques Used: Diagnostic Assay

    Fig. 7 Co-expressed genes ofAP4M1in HCC and gene functional analysis ofAP4M1in HCC. (A) AP4M1 co-expressed gene volcano map obtained from Linkedmoics. (B-C) Heat maps of the expression of the top 50 associated genes that were positively and negatively correlated with AP4M1. (D) KEGG analysis. (E) GSEA-Hallmark signaling pathway enrichment analysis
    Figure Legend Snippet: Fig. 7 Co-expressed genes ofAP4M1in HCC and gene functional analysis ofAP4M1in HCC. (A) AP4M1 co-expressed gene volcano map obtained from Linkedmoics. (B-C) Heat maps of the expression of the top 50 associated genes that were positively and negatively correlated with AP4M1. (D) KEGG analysis. (E) GSEA-Hallmark signaling pathway enrichment analysis

    Techniques Used: Functional Assay, Expressing

    Fig. 8 Depletion of AP4M1 inhibits the proliferation, migration and invasion of HCC. (A) The expression of AP4M1 in the HCC cell lines were de tected by western blotting. (B)The transfection efficiency of si-AP4M1 in Hep 3B. (C) The effect of AP4M1 knockdown on cell proliferation was detected by CCK8 assay. (D) Colony formation assay showed AP4M1 knockdown group were significantly less than siCtrl group cells. (E-F) Transwell assay showed migration (E) and invasion ability (F) after AP4M1 depletion
    Figure Legend Snippet: Fig. 8 Depletion of AP4M1 inhibits the proliferation, migration and invasion of HCC. (A) The expression of AP4M1 in the HCC cell lines were de tected by western blotting. (B)The transfection efficiency of si-AP4M1 in Hep 3B. (C) The effect of AP4M1 knockdown on cell proliferation was detected by CCK8 assay. (D) Colony formation assay showed AP4M1 knockdown group were significantly less than siCtrl group cells. (E-F) Transwell assay showed migration (E) and invasion ability (F) after AP4M1 depletion

    Techniques Used: Migration, Expressing, Western Blot, Transfection, Knockdown, CCK-8 Assay, Colony Assay, Transwell Assay

    Fig. 9 Overexpression ofAP4M1can promote the proliferation, invasion and migration of HCC. (A) AP4M1 was overexpressed in 97 H and HepG2 cell lines (B-C) The effect of AP4M1 overexpression on cell proliferation was detected by CCK8 assay. (D-E) Colony formation assay. (F-I) Transwell assay shows migration (F-G) and invasion ability (H-I) after AP4M1 overexpression
    Figure Legend Snippet: Fig. 9 Overexpression ofAP4M1can promote the proliferation, invasion and migration of HCC. (A) AP4M1 was overexpressed in 97 H and HepG2 cell lines (B-C) The effect of AP4M1 overexpression on cell proliferation was detected by CCK8 assay. (D-E) Colony formation assay. (F-I) Transwell assay shows migration (F-G) and invasion ability (H-I) after AP4M1 overexpression

    Techniques Used: Over Expression, Migration, CCK-8 Assay, Colony Assay, Transwell Assay

    Related Articles

    Expressing:

    Article Title: AP4M1 as a prognostic biomarker associated with cell proliferation, migration and immune regulation in hepatocellular carcinoma.
    Article Snippet: The difference is that quantity of 20 µg of total protein was used for western blot analysis.The difference is that quantity of 20 µg of total protein was used for western blot analysis.. Primary antibodies against AP4M1 and Beta Actin were purchased from Proteintech (Proteintech, Wuhan, China).

    Article Title: AP4M1 as a prognostic biomarker associated with cell proliferation, migration and immune regulation in hepatocellular carcinoma
    Article Snippet: The difference is that quantity of 20 µg of total protein was used for western blot analysis.The difference is that quantity of 20 µg of total protein was used for western blot analysis.. Primary antibodies against AP4M1 and Beta Actin were purchased from Proteintech (Proteintech, Wuhan, China).

    Mutagenesis:

    Article Title: AP4M1 as a prognostic biomarker associated with cell proliferation, migration and immune regulation in hepatocellular carcinoma.
    Article Snippet: The difference is that quantity of 20 µg of total protein was used for western blot analysis.The difference is that quantity of 20 µg of total protein was used for western blot analysis.. Primary antibodies against AP4M1 and Beta Actin were purchased from Proteintech (Proteintech, Wuhan, China).

    Article Title: AP4M1 as a prognostic biomarker associated with cell proliferation, migration and immune regulation in hepatocellular carcinoma
    Article Snippet: The difference is that quantity of 20 µg of total protein was used for western blot analysis.The difference is that quantity of 20 µg of total protein was used for western blot analysis.. Primary antibodies against AP4M1 and Beta Actin were purchased from Proteintech (Proteintech, Wuhan, China).

    Diagnostic Assay:

    Article Title: AP4M1 as a prognostic biomarker associated with cell proliferation, migration and immune regulation in hepatocellular carcinoma.
    Article Snippet: The difference is that quantity of 20 µg of total protein was used for western blot analysis.The difference is that quantity of 20 µg of total protein was used for western blot analysis.. Primary antibodies against AP4M1 and Beta Actin were purchased from Proteintech (Proteintech, Wuhan, China).

    Article Title: AP4M1 as a prognostic biomarker associated with cell proliferation, migration and immune regulation in hepatocellular carcinoma
    Article Snippet: The difference is that quantity of 20 µg of total protein was used for western blot analysis.The difference is that quantity of 20 µg of total protein was used for western blot analysis.. Primary antibodies against AP4M1 and Beta Actin were purchased from Proteintech (Proteintech, Wuhan, China).

    Functional Assay:

    Article Title: AP4M1 as a prognostic biomarker associated with cell proliferation, migration and immune regulation in hepatocellular carcinoma.
    Article Snippet: The difference is that quantity of 20 µg of total protein was used for western blot analysis.The difference is that quantity of 20 µg of total protein was used for western blot analysis.. Primary antibodies against AP4M1 and Beta Actin were purchased from Proteintech (Proteintech, Wuhan, China).

    Article Title: AP4M1 as a prognostic biomarker associated with cell proliferation, migration and immune regulation in hepatocellular carcinoma
    Article Snippet: The difference is that quantity of 20 µg of total protein was used for western blot analysis.The difference is that quantity of 20 µg of total protein was used for western blot analysis.. Primary antibodies against AP4M1 and Beta Actin were purchased from Proteintech (Proteintech, Wuhan, China).

    Migration:

    Article Title: AP4M1 as a prognostic biomarker associated with cell proliferation, migration and immune regulation in hepatocellular carcinoma.
    Article Snippet: The difference is that quantity of 20 µg of total protein was used for western blot analysis.The difference is that quantity of 20 µg of total protein was used for western blot analysis.. Primary antibodies against AP4M1 and Beta Actin were purchased from Proteintech (Proteintech, Wuhan, China).

    Article Title: AP4M1 as a prognostic biomarker associated with cell proliferation, migration and immune regulation in hepatocellular carcinoma
    Article Snippet: The difference is that quantity of 20 µg of total protein was used for western blot analysis.The difference is that quantity of 20 µg of total protein was used for western blot analysis.. Primary antibodies against AP4M1 and Beta Actin were purchased from Proteintech (Proteintech, Wuhan, China).

    Western Blot:

    Article Title: AP4M1 as a prognostic biomarker associated with cell proliferation, migration and immune regulation in hepatocellular carcinoma.
    Article Snippet: The difference is that quantity of 20 µg of total protein was used for western blot analysis.The difference is that quantity of 20 µg of total protein was used for western blot analysis.. Primary antibodies against AP4M1 and Beta Actin were purchased from Proteintech (Proteintech, Wuhan, China).

    Article Title: AP4M1 as a prognostic biomarker associated with cell proliferation, migration and immune regulation in hepatocellular carcinoma
    Article Snippet: The difference is that quantity of 20 µg of total protein was used for western blot analysis.The difference is that quantity of 20 µg of total protein was used for western blot analysis.. Primary antibodies against AP4M1 and Beta Actin were purchased from Proteintech (Proteintech, Wuhan, China).

    Transfection:

    Article Title: AP4M1 as a prognostic biomarker associated with cell proliferation, migration and immune regulation in hepatocellular carcinoma.
    Article Snippet: The difference is that quantity of 20 µg of total protein was used for western blot analysis.The difference is that quantity of 20 µg of total protein was used for western blot analysis.. Primary antibodies against AP4M1 and Beta Actin were purchased from Proteintech (Proteintech, Wuhan, China).

    Article Title: AP4M1 as a prognostic biomarker associated with cell proliferation, migration and immune regulation in hepatocellular carcinoma
    Article Snippet: The difference is that quantity of 20 µg of total protein was used for western blot analysis.The difference is that quantity of 20 µg of total protein was used for western blot analysis.. Primary antibodies against AP4M1 and Beta Actin were purchased from Proteintech (Proteintech, Wuhan, China).

    Knockdown:

    Article Title: AP4M1 as a prognostic biomarker associated with cell proliferation, migration and immune regulation in hepatocellular carcinoma.
    Article Snippet: The difference is that quantity of 20 µg of total protein was used for western blot analysis.The difference is that quantity of 20 µg of total protein was used for western blot analysis.. Primary antibodies against AP4M1 and Beta Actin were purchased from Proteintech (Proteintech, Wuhan, China).

    Article Title: AP4M1 as a prognostic biomarker associated with cell proliferation, migration and immune regulation in hepatocellular carcinoma
    Article Snippet: The difference is that quantity of 20 µg of total protein was used for western blot analysis.The difference is that quantity of 20 µg of total protein was used for western blot analysis.. Primary antibodies against AP4M1 and Beta Actin were purchased from Proteintech (Proteintech, Wuhan, China).

    CCK-8 Assay:

    Article Title: AP4M1 as a prognostic biomarker associated with cell proliferation, migration and immune regulation in hepatocellular carcinoma.
    Article Snippet: The difference is that quantity of 20 µg of total protein was used for western blot analysis.The difference is that quantity of 20 µg of total protein was used for western blot analysis.. Primary antibodies against AP4M1 and Beta Actin were purchased from Proteintech (Proteintech, Wuhan, China).

    Article Title: AP4M1 as a prognostic biomarker associated with cell proliferation, migration and immune regulation in hepatocellular carcinoma
    Article Snippet: The difference is that quantity of 20 µg of total protein was used for western blot analysis.The difference is that quantity of 20 µg of total protein was used for western blot analysis.. Primary antibodies against AP4M1 and Beta Actin were purchased from Proteintech (Proteintech, Wuhan, China).

    Colony Assay:

    Article Title: AP4M1 as a prognostic biomarker associated with cell proliferation, migration and immune regulation in hepatocellular carcinoma.
    Article Snippet: The difference is that quantity of 20 µg of total protein was used for western blot analysis.The difference is that quantity of 20 µg of total protein was used for western blot analysis.. Primary antibodies against AP4M1 and Beta Actin were purchased from Proteintech (Proteintech, Wuhan, China).

    Article Title: AP4M1 as a prognostic biomarker associated with cell proliferation, migration and immune regulation in hepatocellular carcinoma
    Article Snippet: The difference is that quantity of 20 µg of total protein was used for western blot analysis.The difference is that quantity of 20 µg of total protein was used for western blot analysis.. Primary antibodies against AP4M1 and Beta Actin were purchased from Proteintech (Proteintech, Wuhan, China).

    Transwell Assay:

    Article Title: AP4M1 as a prognostic biomarker associated with cell proliferation, migration and immune regulation in hepatocellular carcinoma.
    Article Snippet: The difference is that quantity of 20 µg of total protein was used for western blot analysis.The difference is that quantity of 20 µg of total protein was used for western blot analysis.. Primary antibodies against AP4M1 and Beta Actin were purchased from Proteintech (Proteintech, Wuhan, China).

    Article Title: AP4M1 as a prognostic biomarker associated with cell proliferation, migration and immune regulation in hepatocellular carcinoma
    Article Snippet: The difference is that quantity of 20 µg of total protein was used for western blot analysis.The difference is that quantity of 20 µg of total protein was used for western blot analysis.. Primary antibodies against AP4M1 and Beta Actin were purchased from Proteintech (Proteintech, Wuhan, China).

    Over Expression:

    Article Title: AP4M1 as a prognostic biomarker associated with cell proliferation, migration and immune regulation in hepatocellular carcinoma.
    Article Snippet: The difference is that quantity of 20 µg of total protein was used for western blot analysis.The difference is that quantity of 20 µg of total protein was used for western blot analysis.. Primary antibodies against AP4M1 and Beta Actin were purchased from Proteintech (Proteintech, Wuhan, China).

    Article Title: AP4M1 as a prognostic biomarker associated with cell proliferation, migration and immune regulation in hepatocellular carcinoma
    Article Snippet: The difference is that quantity of 20 µg of total protein was used for western blot analysis.The difference is that quantity of 20 µg of total protein was used for western blot analysis.. Primary antibodies against AP4M1 and Beta Actin were purchased from Proteintech (Proteintech, Wuhan, China).



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    Proteintech primary antibodies against ap4m1
    Fig. 1 AP4M1is highly expressed in liver cancer. (A) <t>AP4M1</t> expression in various tumors in TCGA dataset. (B) The GEO dataset and TCGA-LIHC dataset download from the BEST database showed that AP4M1 was highly expressed in HCC. (C) AP4M1 protein expression levels obtained from CPTAC database. (D) AP4M1 protein levels from the Pan-cancer proteomics study
    Primary Antibodies Against Ap4m1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+antibodies+against+ap4m1/AP4M1+Antibody/pm37821948-85-0-10
    Average 93 stars, based on 1 article reviews
    primary antibodies against ap4m1 - by Bioz Stars, 2026-09
    93/100 stars
      Buy from Supplier

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    Fig. 1 AP4M1is highly expressed in liver cancer. (A) AP4M1 expression in various tumors in TCGA dataset. (B) The GEO dataset and TCGA-LIHC dataset download from the BEST database showed that AP4M1 was highly expressed in HCC. (C) AP4M1 protein expression levels obtained from CPTAC database. (D) AP4M1 protein levels from the Pan-cancer proteomics study

    Journal: Cancer cell international

    Article Title: AP4M1 as a prognostic biomarker associated with cell proliferation, migration and immune regulation in hepatocellular carcinoma.

    doi: 10.1186/s12935-023-03089-0

    Figure Lengend Snippet: Fig. 1 AP4M1is highly expressed in liver cancer. (A) AP4M1 expression in various tumors in TCGA dataset. (B) The GEO dataset and TCGA-LIHC dataset download from the BEST database showed that AP4M1 was highly expressed in HCC. (C) AP4M1 protein expression levels obtained from CPTAC database. (D) AP4M1 protein levels from the Pan-cancer proteomics study

    Article Snippet: Primary antibodies against AP4M1 and Beta Actin were purchased from Proteintech (Proteintech, Wuhan, China).

    Techniques: Expressing

    Fig. 3 Prognostic value ofAP4M1in HCC. (A) Overall survival analysis of AP4M1 mRNA high and low expression in HCC. (B) DSS analysis of AP4M1 mRNA high and low expression in HCC (C)PFS analysis of AP4M1 mRNA high and low expression in HCC (D) RFS analysis of AP4M1 mRNA high and low expres sion in HCC. (E) Survival curves of high and low AP4M1 expression in the GSE54236. (F) Time-dependent ROC curve. (G) Univariate and (H) multivariate COX regression analysis of OS correlation in HCC. OS: overall survival; PFS: Progression Free Survival; RFS: Recurrence Free Survival; DSS: Disease Specific Survival; HCC: hepatocellular carcinoma

    Journal: Cancer cell international

    Article Title: AP4M1 as a prognostic biomarker associated with cell proliferation, migration and immune regulation in hepatocellular carcinoma.

    doi: 10.1186/s12935-023-03089-0

    Figure Lengend Snippet: Fig. 3 Prognostic value ofAP4M1in HCC. (A) Overall survival analysis of AP4M1 mRNA high and low expression in HCC. (B) DSS analysis of AP4M1 mRNA high and low expression in HCC (C)PFS analysis of AP4M1 mRNA high and low expression in HCC (D) RFS analysis of AP4M1 mRNA high and low expres sion in HCC. (E) Survival curves of high and low AP4M1 expression in the GSE54236. (F) Time-dependent ROC curve. (G) Univariate and (H) multivariate COX regression analysis of OS correlation in HCC. OS: overall survival; PFS: Progression Free Survival; RFS: Recurrence Free Survival; DSS: Disease Specific Survival; HCC: hepatocellular carcinoma

    Article Snippet: Primary antibodies against AP4M1 and Beta Actin were purchased from Proteintech (Proteintech, Wuhan, China).

    Techniques: Expressing

    Fig. 4 AP4M1 genetic alterations in HCC. (A) Types of AP4M1 mutation in HCC. (B) AP4M1 alteration was associated with overall survival in HCC. (C) AP4M1 alteration was associated with disease-free survival in HCC. (D) Somatic landscape of HCC in AP4M1-high and AP4M1-low subgroup

    Journal: Cancer cell international

    Article Title: AP4M1 as a prognostic biomarker associated with cell proliferation, migration and immune regulation in hepatocellular carcinoma.

    doi: 10.1186/s12935-023-03089-0

    Figure Lengend Snippet: Fig. 4 AP4M1 genetic alterations in HCC. (A) Types of AP4M1 mutation in HCC. (B) AP4M1 alteration was associated with overall survival in HCC. (C) AP4M1 alteration was associated with disease-free survival in HCC. (D) Somatic landscape of HCC in AP4M1-high and AP4M1-low subgroup

    Article Snippet: Primary antibodies against AP4M1 and Beta Actin were purchased from Proteintech (Proteintech, Wuhan, China).

    Techniques: Mutagenesis

    Fig. 5 Association betweenAP4M1and immune cell infiltration in HCC. (A) AP4M1 is correlated with immune infiltration in HCC. (B-M) According to different expression levels of AP4M1, the infiltration levels of immune cells were analyzed in groups. (N) The relationship between the altered somatic copy number of AP4M1 gene and infiltrating immune cells in HCC. (O) The expression of AP4M1 in immune subtypes of liver cancer. (* p < 0.05; ** p < 0.01; *** p < 0.001, ns, no significance)

    Journal: Cancer cell international

    Article Title: AP4M1 as a prognostic biomarker associated with cell proliferation, migration and immune regulation in hepatocellular carcinoma.

    doi: 10.1186/s12935-023-03089-0

    Figure Lengend Snippet: Fig. 5 Association betweenAP4M1and immune cell infiltration in HCC. (A) AP4M1 is correlated with immune infiltration in HCC. (B-M) According to different expression levels of AP4M1, the infiltration levels of immune cells were analyzed in groups. (N) The relationship between the altered somatic copy number of AP4M1 gene and infiltrating immune cells in HCC. (O) The expression of AP4M1 in immune subtypes of liver cancer. (* p < 0.05; ** p < 0.01; *** p < 0.001, ns, no significance)

    Article Snippet: Primary antibodies against AP4M1 and Beta Actin were purchased from Proteintech (Proteintech, Wuhan, China).

    Techniques: Expressing

    Fig. 6 The correlation analysis ofAP4M1expression and immune checkpoint genes. (A) Immunostimulatory factors (B)Immunosuppressive factors. (C-G) Diagnostic value of AP4M1 for differentiating immunotherapy responses in an immunotherapy cohort. (* p < 0.05; ** p < 0.01; *** p < 0.001, ns, no significance)

    Journal: Cancer cell international

    Article Title: AP4M1 as a prognostic biomarker associated with cell proliferation, migration and immune regulation in hepatocellular carcinoma.

    doi: 10.1186/s12935-023-03089-0

    Figure Lengend Snippet: Fig. 6 The correlation analysis ofAP4M1expression and immune checkpoint genes. (A) Immunostimulatory factors (B)Immunosuppressive factors. (C-G) Diagnostic value of AP4M1 for differentiating immunotherapy responses in an immunotherapy cohort. (* p < 0.05; ** p < 0.01; *** p < 0.001, ns, no significance)

    Article Snippet: Primary antibodies against AP4M1 and Beta Actin were purchased from Proteintech (Proteintech, Wuhan, China).

    Techniques: Diagnostic Assay

    Fig. 7 Co-expressed genes ofAP4M1in HCC and gene functional analysis ofAP4M1in HCC. (A) AP4M1 co-expressed gene volcano map obtained from Linkedmoics. (B-C) Heat maps of the expression of the top 50 associated genes that were positively and negatively correlated with AP4M1. (D) KEGG analysis. (E) GSEA-Hallmark signaling pathway enrichment analysis

    Journal: Cancer cell international

    Article Title: AP4M1 as a prognostic biomarker associated with cell proliferation, migration and immune regulation in hepatocellular carcinoma.

    doi: 10.1186/s12935-023-03089-0

    Figure Lengend Snippet: Fig. 7 Co-expressed genes ofAP4M1in HCC and gene functional analysis ofAP4M1in HCC. (A) AP4M1 co-expressed gene volcano map obtained from Linkedmoics. (B-C) Heat maps of the expression of the top 50 associated genes that were positively and negatively correlated with AP4M1. (D) KEGG analysis. (E) GSEA-Hallmark signaling pathway enrichment analysis

    Article Snippet: Primary antibodies against AP4M1 and Beta Actin were purchased from Proteintech (Proteintech, Wuhan, China).

    Techniques: Functional Assay, Expressing

    Fig. 8 Depletion of AP4M1 inhibits the proliferation, migration and invasion of HCC. (A) The expression of AP4M1 in the HCC cell lines were de tected by western blotting. (B)The transfection efficiency of si-AP4M1 in Hep 3B. (C) The effect of AP4M1 knockdown on cell proliferation was detected by CCK8 assay. (D) Colony formation assay showed AP4M1 knockdown group were significantly less than siCtrl group cells. (E-F) Transwell assay showed migration (E) and invasion ability (F) after AP4M1 depletion

    Journal: Cancer cell international

    Article Title: AP4M1 as a prognostic biomarker associated with cell proliferation, migration and immune regulation in hepatocellular carcinoma.

    doi: 10.1186/s12935-023-03089-0

    Figure Lengend Snippet: Fig. 8 Depletion of AP4M1 inhibits the proliferation, migration and invasion of HCC. (A) The expression of AP4M1 in the HCC cell lines were de tected by western blotting. (B)The transfection efficiency of si-AP4M1 in Hep 3B. (C) The effect of AP4M1 knockdown on cell proliferation was detected by CCK8 assay. (D) Colony formation assay showed AP4M1 knockdown group were significantly less than siCtrl group cells. (E-F) Transwell assay showed migration (E) and invasion ability (F) after AP4M1 depletion

    Article Snippet: Primary antibodies against AP4M1 and Beta Actin were purchased from Proteintech (Proteintech, Wuhan, China).

    Techniques: Migration, Expressing, Western Blot, Transfection, Knockdown, CCK-8 Assay, Colony Assay, Transwell Assay

    Fig. 9 Overexpression ofAP4M1can promote the proliferation, invasion and migration of HCC. (A) AP4M1 was overexpressed in 97 H and HepG2 cell lines (B-C) The effect of AP4M1 overexpression on cell proliferation was detected by CCK8 assay. (D-E) Colony formation assay. (F-I) Transwell assay shows migration (F-G) and invasion ability (H-I) after AP4M1 overexpression

    Journal: Cancer cell international

    Article Title: AP4M1 as a prognostic biomarker associated with cell proliferation, migration and immune regulation in hepatocellular carcinoma.

    doi: 10.1186/s12935-023-03089-0

    Figure Lengend Snippet: Fig. 9 Overexpression ofAP4M1can promote the proliferation, invasion and migration of HCC. (A) AP4M1 was overexpressed in 97 H and HepG2 cell lines (B-C) The effect of AP4M1 overexpression on cell proliferation was detected by CCK8 assay. (D-E) Colony formation assay. (F-I) Transwell assay shows migration (F-G) and invasion ability (H-I) after AP4M1 overexpression

    Article Snippet: Primary antibodies against AP4M1 and Beta Actin were purchased from Proteintech (Proteintech, Wuhan, China).

    Techniques: Over Expression, Migration, CCK-8 Assay, Colony Assay, Transwell Assay